hIL1RL1

Fig1. Detection of IL1RL1 expression in large intestine by RT-PCR in hIL1RL1 mice. 

Wild type: only one band at 296 bp with primers F1/R1(mIl1rl1);

Homoygous: only one band at 367 bp with primers F2/R2(hIL1RL1);

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig.2 Exprssion analysis of of hIL1RL1 on Treg cells in spleen in male hIL1RL1/hTSLP (NM-XA-241614) mice, which were generated by crossing hTSLP(NM-HU-2000064) with hIL1RL1(NM-HU-2000067).

Leukocytes were collected from the spleens of 9-week-old wild-type C57BL/6 mice (WT) and hIL1RL1/hTSLP mice, and analyzed by flow cytometry with anti-hIL1RL1 antibody and anti-mIL1RL1 antibody. hIL1RL1 was exclusively detectable on Treg in hIL1RL1/hTSLP mice, whereas mIL1RL1 was exclusively detectable on Treg in wild-type C57BL/6 mice.

Fig.3 Expression of hIL1RL1 on FcεRIα+ cells in Bone marrow in male hIL1RL1/hTSLP (NM-XA-241614) mice, which were generated by crossing hTSLP(NM-HU-2000064) with hIL1RL1(NM-HU-2000067).

Leukocytes were collected from the bone marrow of 9-week-old wild-type C57BL/6 mice (WT) and hIL1RL1/hTSLP mice, and analyzed by flow cytometry with anti-hIL1RL1 antibody and anti-mIL1RL1 antibody. hIL1RL1 was exclusively detectable on FcεRIα+ cells in hIL1RL1/hTSLP mice, whereas mIL1RL1 was exclusively detectable on FcεRIα+ cells in wild-type C57BL/6 mice.

Fig.4 Detection of human ST2 expression in serum of WT C57BL/6 mice and HO hIL1RL1 mice by ELISA. Human ST2 was detectable in homozygous hIL1RL1 knockin mice. Serum was collected from wild-type C57BL/6 mice (female, 5 weeks old) and HO hIL1RL1 mice (female, 5 weeks old) before or 6h after intraperitoneal injection of human IL-33 and/or mouse IL-33. Human ST2 levels were measured by ELISA with anti-hST2 ELISA kit. 

Abbr.  HO, homozygous; WT, wild type; N.D. not detected.

Fig.5 Detection of mouse ST2 expression in serum of WT C57BL/6 mice and HO hIL1RL1 mice by ELISA. Mouse ST2 was detectable in wild-type C57BL/6 mice but not in hIL1RL1 mice. Serum were collected from wild-type C57BL/6 mice (female, 5 weeks old) and HO hIL1RL1 mice (female, 5 weeks old), and analyzed by ELISA with anti-mST2 ELISA kit. 

Abbr.  HO, homozygous; WT, wild type; N.D. not detected.

Fig.6 Detection of mouse IL5 expression in serum of WT C57BL/6 mice and HO hIL1RL1 mice by ELISA.  Serum were collected from wild-type C57BL/6 mice (female, 5 weeks old) and HO hIL1RL1 mice (female, 5 weeks old) before or 6h after Intraperitoneal injection of  0.025 mg/kg human and/mouse IL33, and analyzed by ELISA with anti-mIL5 ELISA kit. 

Abbr.  HO, homozygous; WT, wild type; N.D. not detected.

Fig.7 Detection of mouse IL13 expression in serum of WT C57BL/6 mice and HO hIL1RL1 mice by ELISA. Serum were collected from wild-type C57BL/6 mice (female, 5 weeks old) and HO hIL1RL1 mice (female, 5 weeks old) before or 6h after Intraperitoneal injection of  0.025 mg/kg human and/mouse IL33, and analyzed by ELISA with anti-mIL13 ELISA kit. 

Abbr.  HO, homozygous; WT, wild type; N.D. not detected.