hIDO1

Fig.1 Detection of IDO1 expression in the thymus of WT C57BL/6 mice (male, 8-10 weeks old) and hIDO1 knockin mice (male, 9 weeks old) by RT-PCR. 

Wild type: one band at 165 bp with primers F1/R1(mIdo1);

Homozygous:  only one band at 112 bp with primers F2/R2(hIDO1).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human IDO1 expression in WT C57BL/6 mice (male, 20 weeks old) and hIDO1 knockin mice (male, 19 weeks old) by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous.

Note. Mice were injected intraperitoneally with mouse IFN-γ (1.25 mg/kg) and sacrificed at 4 h or 48 h post-injection. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.

Fig.3 Detection of human IDO1 expression in splenocytes from WT C57BL/6 mice (female, 8 weeks old) and hIDO1 knockin mice (female, 24 weeks old) by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous; mIFNγ: mouse IFNγ.

Note. “+ “and “−” indicate that splenocytes were cultured with or without mouse IFN-γ (50 ng/mL) for 16 h in vitro, respectively.