hBCMA
Nomenclature
C57BL/6JSmo-Tnfrsf17tm1(hBCMA)Smoc
Cat. NO.
NM-HU-200228
Strain State
Repository Live
Gene Summary
Gene Symbol
Tnfrsf17
Model Description
Validation Data
Fig.1 Detection of BCMA expression in small intestine and spleen by RT-PCR.
Wild type: only one band at 137 bp with primers F1/R1 (mBcma);
Homozygous: only one band at 361 bp with primers F2/R2 (hBCMA).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig.2 Detection of hBCMA expression on TACI+CD138+ cells in spleen of WT C57BL/6 mice and HO hBCMA mice by FACS. Leukocytes were collected from wild-type C57BL/6 mice (male, 11 weeks old) and homozygous hBCMA humanized mice (female, 12 weeks old), and analyzed by flow cytometry with anti-hBCMA. hBCMA was exclusively detectable in hBCMA humanized mice but not in wild-type mice. hBCMA was exclusively detectable on TACI+CD138+ cells in hBCMA humanized mice.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of hBCMA expression on TACI+CD138+ cells in bone marrow of WT C57BL/6 mice and HO hBCMA mice by FACS. Leukocytes were collected from wild-type C57BL/6 mice (male, 11 weeks old) and homozygous hBCMA humanized mice (female,12 weeks old), and analyzed by flow cytometry with anti-hBCMA. hBCMA was exclusively detectable in hBCMA humanized mice but not in wild-type mice. hBCMA was exclusively detectable on TACI+CD138+ cells in hBCMA humanized mice.
Abbr. HO, homozygous; WT, wild type.
Fig.4 Frequency of leukocyte and myeloid subpopulations in the blood by flow cytometry. Blood was collected from WT C57BL/6 mice and HO hBCMA mice (12-week-old, n=4). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. hBCMA knockin mice showed a normal immune phenotype in blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Fig.5 Frequency of leukocyte and myeloid subpopulations in spleen by flow cytometry. Spleen was collected from WT C57BL/6 mice and HO hBCMA mice (12-week-old, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the spleen. hBCMA knockin mice showed a normal immune phenotype in spleen. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
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