hCD20(2)

Extracellular and transmembrane region of the endogenous mouse Ms4a1 gene was replaced by corresponding region of human CD20 gene. While hCD20(Stock No.NM-HU-190068) mice function similarly to hCD20(2) mice,for more detailed information please contact our technical advisor.

Fig1. Detection of hCD20 expression on mCD19+ B cells in blood in hCD20 knockin mice.

Fig 2.Frequency of leukocyte subpopulations in the blood by flow cytometry

Blood was collected from WT C57BL/6 mice(11-week-old, female, n=3) and HO hCD20(2) mice (10-week-old, female, n=5). Flow cytometry analysis of frequencies of lymphocyte subpopulations in the blood. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig 3.Frequency of leukocyte subpopulations in the blood by flow cytometry

Blood was collected from WT C57BL/6 mice(11-week-old, male, n=3) and HO hCD20(2) mice (10-week-old, male, n=5). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig 4.Frequency of leukocyte and myeloid subpopulations in the spleen by flow cytometry

Spleen was collected from WT C57BL/6 mice(11-week-old, female, n=3) and HO hCD20(2) mice (10-week-old, female, n=5). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig 5.Frequency of leukocyte and myeloid subpopulations in the spleen by flow cytometry

Spleen was collected from WT C57BL/6 mice(11-week-old, male, n=3) and HO hCD20(2) mice (10-week-old, male, n=5). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. Bar indicated expressed as mean ± SEM.

Abbr.  HO, homozygous; WT, wild type.

Fig6. Rituximab can bind to lymphocytes of hCD20 knockin mice.

Fig.7 In vivo B cells depletion in hCD20 knockin mice. (In cooperation with a third party)