hFGFR3-G380R

Nomenclature

C57BL/6JSmo-Fgfr3tm1(hFGFR3-G380R)Smoc

Cat. NO.

NM-HU-232149

Strain State

Repository Live

Export PDF

Gene Summary

Gene Symbol
Fgfr3

Synonyms

FR3; Mfr3; sam3; CD333; Flg-2; HBGFR; Fgfr-3

NCBI ID

14184

MGI ID

95524

Ensembl ID

ENSMUSG00000054252

Pubmed

Fgfr3

Human Ortholog

FGFR3

Model Description

The endogenous mouse Fgfr3 gene was replaced by human FGFR3-G380R gene.

Validation Data

image.png

Fig.1 Detection of FGFR3 expression in knee joint and brain in 2.4-week-old male and female hFGFR3-G380R knockin mice by RT-PCR. 

HepG2 cells were used as the WT control of human FGFR3 RNA. 

Wild type: one band at 217 bp with primers F1/R1(mFgfr3) and one band at 123 bp with primers F/R(mGapdh);

Heterozygous: one band at 217 bp with primers F1/R1(mFgfr3), one band at 375 bp with primers F2/R2(hFGFR3) and one band at 123 bp with primers F/R(mGapdh). 

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

6dc74f38-3da2-4bf5-b1de-e043a21464dc.png

Fig.2 Sequencing analysis of human FGFR3 mRNA in HepG2 cells and hFGFR3-G380R knockin mice. 

Sequencing data of RT-PCR products confirmed the presence of a G to A mutation in human FGFR3 mRNA expressed in hFGFR3-G380R knockin mice. 

Abbr. HE, heterozygous.

image.png

Fig.3 Detection of human FGFR3 expression in 2.4-week-old male and female hFGFR3-G380R knockin mice by WB. 

Abbr. M, marker; WT, wild type; HE, heterozygous; PC, positive control, HepG2 cells.

Note. The anti-human FGFR3 antibody cross-reacted with mouse FGFR3 and humanized FGFR3.

image.png

Fig.4 Body weight of WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice. (n=1)

image.png

Fig.5 Micro-CT analysis of skeleton in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

image.png

Fig.6 Micro-CT analysis of skeleton in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

image.png

Fig.7 Micro-CT analysis of skeleton in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

image.png

Fig.8 Micro-CT analysis of skeleton in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).

image.png

Fig.9 Micro-CT analysis of femur in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

image.png

Fig.10 Micro-CT analysis of femur in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

image.png

Fig.11 Micro-CT analysis of femur in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

image.png

Fig.12 Micro-CT analysis of femur in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).

image.png

Fig.13 Micro-CT analysis of skull in 35-day-old  WT C57BL/6 and heterozygous hFGFR3-G380R mice.

image.png

Fig.14 Micro-CT analysis of skull in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice.

image.png

Fig.15 Micro-CT analysis of skull in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice.

image.png

Fig.16 Micro-CT analysis of skull in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).

You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more

Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

Learn more

Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

Learn more