hEGFR

Nomenclature

C57BL/6JSmo-Egfrtm1(hEGFR)Smoc

Cat. NO.

NM-HU-200227

Strain State

Repository Live

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Gene Summary

Gene Symbol
Egfr

Model Description

The endogenous mice Egfr gene was replaced by human EGFR gene. While hEGFR(2)(Stock No.NM-HU-200232) mice have been pulled from shelves for some reasons.

Validation Data

image.png

Fig.1 Detection of EGFR expression in liver and mammary gland by RT-PCR. (male in both groups)

Wild type: only one band at 199 bp with primers F1/R1(mEgfr);

Homozygous:  only one band at 285 bp with primers F2/R2(hEGFR).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of human EGFR expression in hEGFR knockin mice by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control, MCF-7 cell.

Note. The anti-human EGFR antibody cross-reacted with mouse EGFR and humanized EGFR.


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