Il1b-Luc-2A-EGFP

Nomenclature

C57BL/6JSmo-Il1bem1(Luc-eGFP)Smoc

Cat. NO.

NM-KI-00010

Strain State

Repository Live

Export PDF

Gene Summary

Gene Symbol
IL1B

Synonyms

Il-1b; IL-1beta

NCBI ID

16176

MGI ID

96543

Ensembl ID

ENSMUSG00000027398

Pubmed

IL1B

Human Ortholog

IL1B

Model Description

A Luciferase-2A-EGFP-pA cassette was inserted at ATG site of mouse Il1b gene. The intrisic promoter drives the expression of reporter genes, which reflects the activity of Il1b.
Research Application：Inflammation and immunity,gene tracing

Validation Data

微信截图_20211122150850.png

IL1B-2.png

Fig1. IL1β reporter gene expression in the mice was monitored through noninvasive molecular imaging. IL1 β  in the mouse body was activated through LPS  treatment.

2.png

Fig2. Analysis of EGFP expresssion in PBMC by FACS. The homozygous KI mouse expresses EGFP in  F4/80+ macrophages in peripheral blood.

3.png

Fig3. Analysis of EGFP expresssion in the spleen by FACS. The homozygous KI mouse expresses EGFP in F4/80+ macrophages in the spleen.

4.png

Fig4. Analysis of EGFP expresssion in the liver by FACS. The homozygous KI mouse expresses EGFP in F4/80+ macrophages in the liver.


Publications 2piece

1. Carfilzomib modulates tumor microenvironment topotentiate immune checkpoint therapy for cancer
Published year：2021 References：Pub Med

2. Acute pancreatic beta cell apoptosis by IL-1β is responsible for postburn hyperglycemia: Evidence from humans and mice
Published year：2018 References：BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

展开

You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more

Workshop：Progress and Advances in Preclinical immuno-Oncology Research

SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.

Learn more

Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

Learn more