hGRB2

Fig.1 Detection of GRB2 expression in cerebellum and frontal lobe by RT-PCR. 

Tissue RNA was extracted from male 9-week-old wild-type C57BL/6 mice (WT) and homozygous hGRB2 knockin mice (HO), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse- or human- specific primers. Mouse Grb2 mRNA (149 bp) was detectable only in wild-type C57BL/6 mice. Human GRB mRNA (188 bp) was detectable only in homozygous hGRB knockin mice but not in wild-type mice.  

Fig.2 Detection of GRB2 expression in hGRB2 knockin mice by WB. 

Tissues lysates were collected from wild-type C57BL/6 mice (WT, male, 9 weeks old) and homozygous hGRB2 mice (HO, male, 9 weeks old), and then analyzed by western blot with anti-GRB2 antibody. 20 μg of total proteins were loaded for western blotting analysis. Human GRB2 protein is detectable in kidney, lung and bone marrow tissues in GRB2 humanized mice. The antibody is cross-reactive between human and mouse GRB2. The positive control used is Hela cell line.