hTGFBR2
Nomenclature
C57BL/6JSmo-Tgfbr2tm1(hTGFBR2)Smoc
Cat. NO.
NM-HU-252006
Strain State
Repository Live
Gene Summary
Gene Symbol
Tgfbr2
Model Description
Validation Data
Fig.1 Detection of TGFBR2 expression in subcutaneous fat pad and lung by RT-PCR. Mouse Tgfbr2 mRNA (145 bp) was detectable only in wild-type C57BL/6 mice. Human TGFBR2 mRNA (319 bp/429 bp) was detectable only in homozygous hTGFBR2 knockin mice but not in wild-type mice. Subcutaneous fat pad and lung RNA was extracted from 7-week-old male wild-type C57BL/6 (WT)(n=1) and homozygous hTGFBR2 knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 Detection of human TGFBR2 expression on B cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (male,7 weeks old) and homozygous hTGFBR2 humanized mice (male,7 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of mouse TGFBR2 expression on B cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (male,7 weeks old) and homozygous hTGFBR2 humanized mice (male,7 weeks old), and analyzed by flow cytometry with anti-mTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.4 Detection of human TGFBR2 expression on T cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (male,7 weeks old) and homozygous hTGFBR2 humanized mice (male,7 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.5 Detection of mouse TGFBR2 expression on T cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (male,7 weeks old) and homozygous hTGFBR2 humanized mice (male,7 weeks old), analyzed by flow cytometry with anti-mTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.6 Detection of human TGFBR2 expression on CD4+T cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (male,7 weeks old) and homozygous hTGFBR2 humanized mice (male,7 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.7 Detection of mouse TGFBR2 expression on CD4+T cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (male,7 weeks old) and homozygous hTGFBR2 humanized mice (male,7 weeks old), and analyzed by flow cytometry with anti-mTGFBR2.
Abbr. HO, homozygous; WT, wild type.
You may also like
Workshop：Progress and Advances in Preclinical immuno-Oncology Research
SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.
Learn moreShanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn more
