hAQP2

Fig.1 Detection of AQP2 expression in kidney by RT-PCR. Kidney RNA was extracted from 3-4-week-old male wild-type C57BL/6 (WT) and heterozygous hAQP2 knockin mice (HE), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Aqp2 mRNA (142 bp) was detectable both in wild-type C57BL/6 mice and heterozygous hAQP2 knockin mice. Human AQP2 mRNA (256 bp) was detectable only in heterozygous hAQP2 knockin mice but not in wild-type mice. 

Abbr. M, marker; HE, heterozygous; WT, wild type.

Fig.2 Immunohistochemistry staining in hAQP2 and WT C57BL/6 mice. Immunohistochemistry (IHC) analysis demonstrated that, compared to the isotype control, human-derived AQP2 was expressed in kidney tissues. Additionally, antibody cross-reactivity was detected concurrently. Kidneys tissues were collected from mice and subjected to IHC staining using an anti-human AQP2 antibody(3-4 weeks old, n=1). Scale bar, 50 μm.

Abbr. HE, heterozygous; WT, wild type.