hCCN4(2)

Nomenclature

C57BL/6JSmo-Ccn4tm1(hCCN4)Smoc

Cat. NO.

NM-HU-253774

Strain State

Repository Live

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Gene Summary

Gene Symbol
Ccn4

Model Description

The endogenous mouse Ccn4 gene was replaced by human CCN4 gene.

Validation Data

2d46cea7-acfd-42ea-a365-2f4a3076c250.png

Fig.1 Detection of CCN4 expression in ovary by RT-PCR. Ovary RNA was extracted from 6-week-old female wild-type C57BL/6 (WT)(n=2) and homozygous hCCN4(2) knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

3a342200-3026-481e-b568-2a0f8cf043cc.png

Fig.2 Detection of CCN4 expression in hCCN4(2) mice by WB. The spleen, kidney and lung tissue lysates were collected from 6-week-old female wild-type C57BL/6 mice and 6-week-old female homozygous hCCN4(2) mice, and then analyzed by western blot with anti-CCN4 antibody. 

Abbr. HO, homozygous; WT, wild type.


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