Jak2-Flox-V617F/Vav1-2A-Cre

Fig. 1 Body weight and body weight change curves of HE/HE Jak2-Flox-V617F;Vav1-2A-Cre and WT C57BL/6 mice in a bone marrow adoptive transfer model. WT CD45.1 C57BL/6 recipient mice were lethally irradiated and transplanted with bone marrow from HE/HE Jak2-Flox-V617F;Vav1-2A-Cre, and body weight from all groups was monitored weekly post 2 weeks after transplantation. (A) Absolute body weight. (B) Percentage body weight change relative to baseline.  (n = 3-5 per group, 8 weeks old). Data are presented as mean ± SEM. 

Abbr: WT, wild type; HE, Heterozygous. 

Fig. 2 Complete blood count (CBC) profile of (HE/HE) Jak2-Flox-V617F;Vav1-2A-Cre mice and BM-transplanted model. Peripheral blood parameters from WT,  (HE/HE) Jak2-Flox-V617F;Vav1-2A-Cre mice and BM-transplanted model (3-5/group, 8 week-old), including WBC, RBC,HGB, RDW-CV, NEUT, MONO counts, and LYMPH were measured.  Data are presented as mean ± SEM if available. 

Abbr: Heterozygous, HE; wild type, WT; bone marrow, BM; white blood cell, WBC; red blood cell, RBC; hemoglobin, HGB; red blood cell distribution width-coefficient of variation, RDW-CV; neutrophil, NEUT; monocyte, MONO; lymphocyte, LYMPH; complete blood count, CBC; 

Fig. 3 Spleen size are significantly increased in HE/HE Jak2-Flox-V617F;Vav1-2A-Cre mice and BM transfer model. (A) Representative photographs of spleens from C57BL/6 WT, HE/HE Jak2-Flox-V617F;Vav1-2A-Cre mice and its BM-transferred mice.  

Abbr: heterozygous, HE/HE; transgenic, Tg; wild type, WT.

Fig. 4 Spleen histopathology and injury score. (A) Representative H&E-stained spleen sections. G1 (WT control): normal architecture with intact PALS, lymphoid follicles, and germinal centers(Green arrow). G2 (mutant): marked extramedullary hematopoiesis, red pulp expansion, and no significant white pulp. G3 (Transplanted): moderate extramedullary hematopoiesis with partial white pulp loss and tiny activate residual germinal centers (Green arrow). (B) Spleen injury scores for each group.(n=2)

Fig. 5 Assessment of donor cell engraftment by CD45.1 and CD45.2 flow cytometry at Day 28 post bone marrow transplantation. Peripheral blood was collected from recipient mice, and the frequencies of CD45.1+ and CD45.2+ cells among total CD45+ leukocytes were analyzed. (A) Frequency of CD45.1+ donor-derived cells. (B) Frequency of CD45.2+ recipient-derived cells. n = 3–5 per group. Data are presented as mean ± SEM. 

Abbr. WT, wild type; HE, heterozygous; BM, bone marrow.

Fig. 5 Flow cytometric analysis of erythroid precursors and myeloid cells in peripheral blood of WT C57BL/6, HE/HE Jak2-Flox-V617F;Vav1-2A-Cre and BM-recipient mice at Day 28 post bone marrow transplantation. Peripheral blood was collected and analyzed for (A) TER-119+ erythroid cells, (B) TER-119+CD71+ erythroid precursors, (C) CD11b+Gr-1+ myeloid cells in CD45+ leukocytes, and (D) CD11b+Gr-1+ myeloid cells in CD45.2+ donor-derived cells. n = 3–5 per group. Data are presented as mean ± SEM. 

Abbr. WT, wild type; HE, heterozygous; BM, bone marrow.