hPTH

Fig.1 Detection of PTH expression in thymus by RT-PCR. Mouse Pth mRNA (135 bp) was detectable only in wild-type C57BL/6 mice. Human PTH mRNA (146 bp) was detectable only in homozygous hPTH knockin mice but not in wild-type mice. Thymus RNA was extracted from 8-week-old female wild-type C57BL/6 (WT) (n=2) and 8-week-old female homozygous hPTH knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human PTH expression in thyroid glands of WT C57BL/6 mice and HO hPTH mice by ELISA. Human PTH was detectable in thyroid glands (containing parathyroid glands) from both WT C57BL/6 and HO hPTH knockin mice, as the ELISA kit was cross-reactived between human and mouse. Lysates of thyroid glands (containing parathyroid glands) were collected from wild-type C57BL/6 mice (n=3, male, 8 weeks old) and HO hPTH mice (n=3, male, 7 weeks old), and analyzed by ELISA. 

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of human PTH expression in serum of WT C57BL/6 mice and HO hPTH mice by ELISA. Human PTH was detectable in serum from both WT C57BL/6 and HO hPTH mice, as the ELISA kit was cross-reactived between human and mouse. Serum were collected from wild-type C57BL/6 mice (n=3, female, 8 weeks old) and HO hPTH mice (n=3, female, 7 weeks old), and analyzed by ELISA. 

Abbr.  HO, homozygous; WT, wild type.