hTYRO3

Fig.1 Detection of TYRO3 expression in cortex, cerebellum and frontal lobe by RT-PCR. Mouse Tyro3 mRNA (382 bp) was detectable only in wild-type C57BL/6 mice. Human TYRO3 mRNA (349 bp) was detectable only in homozygous hTYRO3 knockin mice but not in wild-type mice. RNA of cortex, cerebellum and frontal lobe tissues was extracted from 6-week-old male WT C57BL/6 (n=2) and homozygous hTYRO3 knockin mice (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of TYRO3 expression in hTYRO3 mice by WB. TYRO3 was detectable in kidney, lung, stomach, testis and brain from both WT C57BL/6 and HO hTYRO3 mice, as the antibody was cross-reactived between human and mouse TYRO3. The kidney, lung, stomach, testis and brain tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and 6-week-old male homozygous hTYRO3 mice, and then analyzed by western blot with anti-human TYRO3 antibody. 

Abbr. HO, homozygous; WT, wild type; M, marker.