hCCR9

Fig.1 Detection of CCR9 expression in thymus by RT-PCR. 

Thymus RNA was extracted from male 8-10-week-old wild-type C57BL/6 mice (WT) and heterozygous hCCR9 knockin mice (HE), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human mRNA primers. Mouse Ccr9 mRNA (188 bp) was detectable in all mice. Human CCR9 mRNA (164 bp) was detectable only in heterozygous hCCR9 knockin mice but not in wild-type mice. 

Fig.2 Detection of CCR9 expression on T cells in thymus of WT C57BL/6 mice and HO hCCR9 mice by FACS.  hCCR9 was exclusively detectable in hCCR9 humanized mice but not in wild-type mice. hCCR9 was exclusively detectable on T cells in hCCR9 humanized mice, whereas mCCR9 was exclusively detectable on T cells in wild-type C57BL/6 mice. Leukocytes were collected from thymus of wild-type C57BL/6 mice (female, 9 weeks old) and homozygous hCCR9 humanized mice (female, 9 weeks old), and analyzed by flow cytometry with anti-mCCR9 and anti-hCCR9. 

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of CCR9 expression on T cells in spleen of WT C57BL/6 mice and HO hCCR9 mice by FACS. hCCR9 was exclusively detectable in hCCR9 humanized mice but not in wild-type mice. hCCR9 was exclusively detectable on T cells in hCCR9 humanized mice, whereas mCCR9 was exclusively detectable on T cells in wild-type C57BL/6 mice. Leukocytes were collected from spleen of wild-type C57BL/6 mice (female, 9 weeks old) and homozygous hCCR9 humanized mice (female, 9 weeks old), and analyzed by flow cytometry with anti-mCCR9  and anti-hCCR9. 

Abbr. HO, homozygous; WT, wild type.