F12-KO
Nomenclature
C57BL/6JSmo-F12em1Smoc
Cat. NO.
NM-KO-200193
Strain State
Embryo Cryopreservation
Gene Summary
Gene Symbol
F12
Model Description
Validation Data
Fig.1 Detection of F12 expression in liver by RT-PCR. Mouse F12 mRNA (214 bp/136 bp) was detectable only in wild-type C57BL/6 mice. Mouse Gapdh mRNA (123 bp) was detectable both in wild-type C57BL/6 mice and homozygous F12-KO mice. Liver RNA was extracted from 7-week-old female wild-type C57BL/6 (WT) (n=2) and 6-week-old female homozygous F12-KO mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 Detection of F12 expression in liver by qPCR. Mouse F12 mRNA was detectable only in wild-type mice but not in homozygous F12-KO mice.Liver RNA was extracted from 7-week-old female wild-type C57BL/6 (WT) (n=2) and 6-week-old female homozygous F12-KO mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse F12 and mouse Gapdh primers. Relative expression represents the mouse F12 mRNA level relative to its average expression in wild-type mice .
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of mouse F12 expression in serum of WT C57BL/6 mice and HO F12-KO mice by ELISA. Mouse F12 was exclusively detectable in wild-type C57BL/6 mice. Serum was collected from wild-type C57BL/6 mice (n=2, female, 7 weeks old) and HO F12-KO mice (n=2, female, 6 weeks old), and analyzed by ELISA with anti-mF12 ELISA kit .
Abbr. HO, homozygous; WT, wild type; N.D. not detected.
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