Fus-R513C
Nomenclature
C57BL/6JSmo-Fusem1(R513C)Smoc
Cat. NO.
NM-KI-232084
Strain State
Repository Live
Gene Summary
Gene Symbol
Fus
Model Description
Validation Data
Fig.1 Detection of Fus expression in brain and spinal cord by RT-PCR. Mouse Fus mRNA (420 bp) and mouse Gapdh mRNA (123 bp) was detectable both in homozygous Fus-R513C knockin mice and wild-type mice. Brain and spinal cord RNA was extracted from 8-week-old male wild-type C57BL/6 (WT)(n=2) and 12-week-old male homozygous Fus-R513C knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 mRNA sequencing of Fus-R513C knockin mice. Sequencing data of RT-PCR products confirmed the presence of target mutations in Fus-R513C knockin mice.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of FUS expression in HO Fus-R513C mice by WB. FUS was detectable in brain and spinal cord from both WT C57BL/6 and HO Fus-R513C mice.The brain and spinal cord lysates were collected from 8-weeks-old wild-type C57BL/6 mice and 12-weeks-old homozygous Fus-R513C mice, and then analyzed by western blot with anti-FUS antibody.
Abbr. HO, homozygous; WT, wild type; PC, positive control, NIH/3T3 cells; M, marker.
You may also like
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreWorkshop：Progress and Advances in Preclinical immuno-Oncology Research
SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn more
