hIL12RB1(5)
Nomenclature
C57BL/6JSmo-Il12rb1tm3(hIL12RB1)Smoc
Cat. NO.
NM-HU-251805
Strain State
Repository Live
Gene Summary
Gene Symbol
Il12rb1
Model Description
Validation Data
Fig.1 Detection of IL12RB1 expression in thymus and spleen by RT-PCR. Mouse Il12rb1 mRNA (150 bp) was detectable only in WT C57BL/6 mice. Human IL12RB1 mRNA (193 bp) was detectable only in HO hIL12RB1(5) knockin mice but not in WT mice. Thymus and spleen RNA was extracted from 10-week-old male wild-type C57BL/6 (WT)(n=2) and homozygous hIL12RB1(5) knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 Detection of IL12RB1 expression in hIL12RB1(5) mice by WB. IL12RB1 was detectable in spleen from both WT C57BL/6 and HO hIL12RB1(5) mice, as the antibody was cross-reactived between human and mouse IL12RB1. The spleen tissue lysates were collected from 10-week-old male WT C57BL/6 mice and 10-week-old male HO hIL12RB1(5) mice, and then analyzed by western blot with anti-IL12RB1 antibody.
Abbr. HO, homozygous; WT, wild type; PC, positive control, MCF-7 cells; M, marker.
Fig.3 Detection of IL12RB1 expression in hIL12RB1(5) mice by WB. IL12RB1 was detectable in spleen from both WT C57BL/6 and HO hIL12RB1(5) mice, as the antibody was cross-reactived between human and mouse IL12RB1. The spleen tissue lysates were collected from 10-week-old male WT C57BL/6 mice and 10-week-old male HO hIL12RB1(5) mice, and then analyzed by western blot with anti-IL12RB1 antibody (The antibody used is different from the one used in Fig.2)
Abbr. HO, homozygous; WT, wild type; PC, positive control, MCF-7 cells; M, marker.
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