hCD69
Nomenclature
C57BL/6JSmo-Cd69tm1(hCD69)Smoc
Cat. NO.
NM-HU-251808
Strain State
Repository Live
Gene Summary
Gene Symbol
Cd69
Model Description
Validation Data
Fig.1 Detection of human CD69 expression on B cells in spleen of WT C57BL/6 mice and HO hCD69 mice by FACS. hCD69 was exclusively detectable in hCD69 humanized mice but not in wild-type mice. hCD69 was exclusively detectable on B cells in hCD69 humanized mice, whereas mCD69 was exclusively detectable on B cells in wild-type C57BL/6 mice. Leukocytes were collected from wild-type C57BL/6 mice (female,8 weeks old) and homozygous hCD69 humanized mice (female,8 weeks old), stimulated with 1μg/ml anti-CD3 and 1μg/ml anti-CD28 for 24 h in vitro and analyzed by flow cytometry with anti-mCD69 and anti-hCD69.
Abbr. HO, homozygous; WT, wild type.
Fig.2 Detection of mouse CD69 expression on B cells in spleen of WT C57BL/6 mice and HO hCD69 mice by FACS. hCD69 was exclusively detectable in hCD69 humanized mice but not in wild-type mice. hCD69 was exclusively detectable on B cells in hCD69 humanized mice, whereas mCD69 was exclusively detectable on B cells in wild-type C57BL/6 mice. Leukocytes were collected from wild-type C57BL/6 mice (female,8 weeks old) and homozygous hCD69 humanized mice (female,8 weeks old), stimulated with 1μg/ml anti-CD3 and 1μg/ml anti-CD28 for 24 h in vitro and analyzed by flow cytometry with anti-mCD69 and anti-hCD69.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of human CD69 expression on T cells in spleen of WT C57BL/6 mice and HO hCD69 mice by FACS. hCD69 was exclusively detectable in hCD69 humanized mice but not in wild-type mice. hCD69 was exclusively detectable on T cells in hCD69 humanized mice, whereas mCD69 was exclusively detectable on T cells in wild-type C57BL/6 mice. Leukocytes were collected from wild-type C57BL/6 mice (female,8 weeks old) and homozygous hCD69 humanized mice (female,8 weeks old), stimulated with 1μg/ml anti-CD3 and 1μg/ml anti-CD28 for 24 h in vitro and analyzed by flow cytometry with anti-mCD69 and anti-hCD69.
Abbr. HO, homozygous; WT, wild type.
Fig.4 Detection of mouse CD69 expression on T cells in spleen of WT C57BL/6 mice and HO hCD69 mice by FACS. hCD69 was exclusively detectable in hCD69 humanized mice but not in wild-type mice. hCD69 was exclusively detectable on T cells in hCD69 humanized mice, whereas mCD69 was exclusively detectable on T cells in wild-type C57BL/6 mice. Leukocytes were collected from wild-type C57BL/6 mice (female,8 weeks old) and homozygous hCD69 humanized mice (female,8 weeks old), stimulated with 1μg/ml anti-CD3 and 1μg/ml anti-CD28 for 24 h in vitro and analyzed by flow cytometry with anti-mCD69 and anti-hCD69.
Abbr. HO, homozygous; WT, wild type.
Fig.5 Detection of human CD69 expression on NK cells in spleen of WT C57BL/6 mice and HO hCD69 mice by FACS. hCD69 was exclusively detectable in hCD69 humanized mice but not in wild-type mice. hCD69 was exclusively detectable on NK cells in hCD69 humanized mice, whereas mCD69 was exclusively detectable on NK cells in wild-type C57BL/6 mice. Leukocytes were collected from wild-type C57BL/6 mice (female,8 weeks old) and homozygous hCD69 humanized mice (female,8 weeks old), stimulated with 1μg/ml anti-CD3 and 1μg/ml anti-CD28 for 24 h in vitro and analyzed by flow cytometry with anti-mCD69 and anti-hCD69.
Abbr. HO, homozygous; WT, wild type.
Fig.6 Detection of mouse CD69 expression on NK cells in spleen of WT C57BL/6 mice and HO hCD69 mice by FACS. hCD69 was exclusively detectable in hCD69 humanized mice but not in wild-type mice. hCD69 was exclusively detectable on NK cells in hCD69 humanized mice, whereas mCD69 was exclusively detectable on NK cells in wild-type C57BL/6 mice. Leukocytes were collected from wild-type C57BL/6 mice (female,8 weeks old) and homozygous hCD69 humanized mice (female,8 weeks old), stimulated with 1μg/ml anti-CD3 and 1μg/ml anti-CD28 for 24 h in vitro and analyzed by flow cytometry with anti-mCD69 and anti-hCD69.
Abbr. HO, homozygous; WT, wild type.
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