hCD5(BALB/c)
Nomenclature
BALB/cJSmo-Cd5tm1(hCD5)Smoc
Cat. NO.
NM-HU-241178
Strain State
Repository Live
Gene Summary
Gene Symbol
Cd5
Model Description
Validation Data
Fig.1 Detection of CD5 expression in thymus by RT-PCR. Mouse Cd5 mRNA (162 bp) was detectable only in wild-type BALB/c mice. Human CD5 mRNA (249 bp) was detectable only in homozygous hCD5 (BALB/c) knockin mice but not in wild-type mice. Thymus RNA was extracted from 7-week-old female wild-type BALB/c (WT)(n=3) and homozygous hCD5 (BALB/c) knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 Detection of CD5 expression on T cells and B cells in spleen of WT (BALB/c) mice and HO hCD5(BALB/c) mice by FACS. hCD5 was exclusively detectable in hCD5(BALB/c) humanized mice but not in wild-type (BALB/c) mice. hCD5 was exclusively detectable on T cells and B cells in hCD5(BALB/c) humanized mice, whereas mCD5 was exclusively detectable on T cells and B cells in wild-type BALB/c mice. Leukocytes were collected from spleen of wild-type BALB/c mice (female, 6 weeks old) and homozygous hCD5(BALB/c) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with anti-mCD5 and anti-hCD5.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of CD5 expression on T cells and B cells in blood of WT (BALB/c) mice and HO hCD5(BALB/c) mice by FACS. hCD5 was exclusively detectable in hCD5(BALB/c) humanized mice but not in wild-type (BALB/c) mice. hCD5 was exclusively detectable on T cells and B cells in hCD5(BALB/c) humanized mice, whereas mCD5 was exclusively detectable on T cells and B cells in wild-type BALB/c mice. Leukocytes were collected from blood of wild-type BALB/c mice (female, 6 weeks old) and homozygous hCD5(BALB/c) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with anti-mCD5 and anti-hCD5.
Abbr. HO, homozygous; WT, wild type.
Fig.4 Frequency of leukocyte and myeloid subpopulations in the blood by flow cytometry. hCD5(BALB/c) knockin mice showed a normal immune phenotype in blood. Blood was collected from WT BALB/c mice and HO hCD5(BALB/c) mice (6-week-old, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Fig.5 Frequency of leukocyte and myeloid subpopulations in the spleen by flow cytometry. hCD5(BALB/c) knockin mice showed a normal immune phenotype in spleen. Spleen was collected from WT BALB/c mice and HO hCD5(BALB/c) mice (6-week-old, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the spleen. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
You may also like
Workshop：Progress and Advances in Preclinical immuno-Oncology Research
SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.
Learn moreShanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreCustomizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn more
