R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg

Nomenclature

C57BL/6JSmo-Gt(ROSA)26Sortm1(SA-Frt-2xpolyA-CAG-Frt-LSL-PNPLA3*I148M-IRES-GFP-wpre-polyA)Tg(Alb-Cre)Smoc

Cat. NO.

NM-XA-242889

Strain State

Repository Live

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Model Description

The R26-CAG-LSL-hPNPLA3-I148M-EGFP was crossed with Alb-cre-Tg to generate R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg mice.

Validation Data

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Fig.1 Detection of human PNPLA3 expression in liver by qPCR. Each value in G1 and G2 represents the mRNA level relative to the value in G3(n=3 for G1 and G3, n=1 for G2).

Abbr. WT, wild type.

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Fig.2 Detection of human PNPLA3 expression in liver by WB. 

Abbr. WT: wild type; HE/+: R26CAG-LSL-hPNPLA3-I148M-EGFP/+; Alb-Cre-Tg. HE/-: R26CAG-LSL-hPNPLA3-I148M-EGFP/+.

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Fig.3 The body weight curve of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice. (n=3-8).

Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.

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Fig.4 Blood chemistry and blood lipid profile in serum of  R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=6-9, male/female).

Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.

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Fig.5 TG and T-CHO in liver tissues of  R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=3-4, male/female).

Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.

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Fig.6 Representative H&E staining images of liver of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=3-4，Each image is from a different mouse). 

Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.

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Fig.7 Representative masson staining images of liver of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=3-4, Each image is from a different mouse). 

Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.

G1: C57BL/6, CD; G2: C57BL/6, HSD1; G3: R26CAG-LSL-hPNPLA3-I148M-EGFP/+; Alb-Cre-Tg, HSD1; G4: R26CAG-LSL-hPNPLA3-I148M-EGFP/CAG-LSL-hPNPLA3-I148M-EGFP; Alb-Cre-Tg, CD; G5: R26CAG-LSL-hPNPLA3-I148M-EGFP/CAG-LSL-hPNPLA3-I148M-EGFP; Alb-Cre-Tg, HSD1

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Fig.8 Representative Oil red O staining images of liver of R26-CAG-LSL-hPNPLA3-I148M-EGFP/Alb-cre-Tg and C57BL/6 mice after 6 weeks post diet feeding. (n=3-4, Each image is from a different mouse). 

Abbr. WT, wild type; CD, chow diet; HSD, high-sugar diet.

G1: C57BL/6, CD; G2: C57BL/6, HSD1; G3: R26CAG-LSL-hPNPLA3-I148M-EGFP/+; Alb-Cre-Tg, HSD1; G4: R26CAG-LSL-hPNPLA3-I148M-EGFP/CAG-LSL-hPNPLA3-I148M-EGFP; Alb-Cre-Tg, CD; G5: R26CAG-LSL-hPNPLA3-I148M-EGFP/CAG-LSL-hPNPLA3-I148M-EGFP; Alb-Cre-Tg, HSD1

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