Humanization strategy of the heavy chain variable region
SmocMab® Fully Humanized Antibody Mouse
SmocMab® fully humanized antibody mice employ large-fragment gene sequence replacement technology to integrate the human antibody variable region gene library into the endogenous loci of C57BL/6 mice. This approach achieves full humanization of the variable region while preserving close-to-wild-type immune response capabilities, delivering a broader spectrum of immune responses and enabling the successful production of large quantities of high-affinity fully humanized antibodies to accelerate therapeutic antibody drug development.
Humanization Strategy
Humanization strategy of the kappa light chain variable region
Immunoassay
Normal B cell development lays a solid foundation for antibody production.
Fully Human Antibody Repertoire Diversity
Case Studies
Fig. Detection of antibody titer in serum after 1st or 2nd boost in SmocMab and C57BL/6.
Fig. Detection of antibody titer in serum after three antigen immunizations in SmocMab(A) and C57BL/6(B).
Fig. Detection of antibody titer in serum after three antigen immunizations in SmocMab and C57BL/6.
Fig. Detection of antibody titer in serum after three antigen immunizations in Smocmab and C57BL/6
Fig. Detection of antibody titer in serum after three antigen immunizations in SmocmabTM(A) and C57BL/6(B).
Fig. C57 mice were injected with different form of F-Luc mRNA intramuscularly. Eight hours after transfection, Mice received Self-Amplifying LNP-delivered F-Luc mRNA exhibited high and prolonged protein expression.
Fig. Detection of antibody titer in serum after three antigen immunizations in protein or mRNA-LNP immunization.
